Tamar Kleinberger Lab
Department of Microbiology
Rappaport Faculty of Medicine
Technion - Israel Institute of Technology
SELECTED PUBLICATIONS
Click on the adenovirus link to see the publication
Brestovitsky A, Nebenzahl-Sharon K, Kechker P, Sharf R, Kleinberger T.
The Adenovirus E4orf4 Protein Provides a Novel Mechanism for Inhibition of the DNA Damage Response.PLoS Pathog. 2016 Feb 11;12(2):e1005420. doi: 10.1371/journal.ppat.1005420.
Kleinberger T.
Mechanisms of cancer cell killing by the adenovirus E4orf4 protein. Viruses. 2015 May 7;7(5):2334-57. doi: 10.3390/v7052334. Review.
Avital-Shacham M, Sharf R, Kleinberger T.
NTPDASE4 gene products cooperate with the adenovirus E4orf4 protein through PP2A-dependent and -independent mechanisms and contribute to induction of cell death. J Virol. 2014 Jun;88(11):6318-28. doi: 10.1128/JVI.00381-14.
Pechkovsky, A., Salzberg, A., and Kleinberger, T.
The adenovirus E4orf4 protein induces a unique mode of cell death while inhibiting classical apoptosis. 2013. Cell Cycle 12.
Pechkovsky, A., Lahav, M., Bitman, E., Salzberg, A., and Kleinberger, T.
E4orf4 induces PP2A- and Src-dependent cell-death in Drosophila melanogaster and at the same time inhibits classical apoptosis pathways. Proc Natl Acad Sci USA 2013 May 7; 110(19):E1724-33. doi: 10.1073/pnas.1220282110. Epub 2013 Apr 23.
Horowitz,B., Sharf,R., Avital-Shacham, M., Pechkovsky, A., and Kleinberger, T.
Structure- and modeling-based identification of the adenovirus E4orf4 binding site in the protein phosphatase 2A B55a subunit. J Biol Chem. 2013 May 10; 288(19):13718-27. doi: 10.1074/jbc.M112.343756. Epub 2013 Mar 25.
Brestovitsky, A., Sharf, R., and Kleinberger, T.
Preparation of cell-lines for conditional knockdown of gene expression and measurement of the knockdown effects on E4orf4-induced cell death. J. Vis. Exp. J Vis Exp. 2012 Oct 21;(68). doi:pii: 4442. 10.3791/4442. (2012).
Brestovitsky, A., Sharf, R., Mittelman, K., and Kleinberger, T.
The adenovirus E4orf4 protein targets PP2A to the ACF chromatin-remodeling factor and induces cell death through regulation of SNF2h-containing complexes. 2011. Nucleic Acids Res. 39, 6414-6427.
Mittelman, K., Ziv, K., Maoz, T., and Kleinberger, T.
The cytosolic tail of the Golgi apyrase Ynd1 mediates E4orf4-induced toxicity in Saccharomyces cerevisiae. 2010. PLOS ONE, 2010 Nov 22;5(11):e15539.
Ben-Israel, H., Sharf, R., Rechavi, G., and Kleinberger, T.
Adenovirus E4orf4 Protein Downregulates MYC Expression through Interaction with the PP2A-B55 Subunit. 2008. J. Virol. 82, 9381-9388.
Maoz, T., Koren, R., Ben-Ari, I., Kleinberger T.
YND1 interacts with CDC55 and is a novel mediator of E4orf4-induced toxicity. 2005. J Biol Chem. 280, 41270-41277.
Koren, R., Rainis, L., and Kleinberger, T.
The scaffolding A/Tpd3 subunit and high phosphatase activity are dispensable for Cdc55 function in the S. cerevisiae spindle checkpoint and in cytokinesis. 2004. J Biol Chem. 279, 48598-48606.
Kleinberger, T.
Induction of transformed cell-specific apoptosis by the adenovirus E4orf4 protein. 2004. Prog. Mol. Subcell. Biol. Springer-Verlag, Heidelberg, Germany. 36, 245-267.
Barr, H.M., Sharf, R., and Kleinberger, T.
Using the Ras recruitment system to identify PP2A-B55 – interacting proteins. 2003. Methods enzymol. 366, 175-187.
Liu, B., Liang, M.H., Kuo, Y.L., Liao, W., Boros, I., Kleinberger, T., Blancato, J., and Giam, C.Z.
Human T-lymphotropic virus type 1 oncoprotein tax promotes unscheduled degradation of Pds1p/securin and Clb2p/cyclin B1 and causes chromosomal instability. 2003. Mol. Cell. Biol. 23, 5269-5281.
Ben-Israel H, and Kleinberger T.
Adenovirus and cell cycle control. 2002. Front Biosci. 7, D1369-D1395.
Kornitzer, D., Sharf, R., and Kleinberger, T.
Adenovirus E4orf4 protein induces PP2A-dependent growth arrest in S. cerevisiae and interacts with the APC/Cyclosome. 2001. J. Cell Biol. 154, 331-344.
Afifi, R., Sharf, R., Shtrichman, R., and Kleinberger, T.
Selection of apoptosis-deficient E4orf4 mutants in S. cerevisiae. 2001. J. Virol 75, 4444-4447.
Livne, A., Shtrichman, R., and Kleinberger, T.
Caspase activation by adenovirus E4orf4 protein is cell line-specific and is mediated by the death receptor pathway. 2001. J. Virol. 75, 789-798.
Nilsson, C.E., Petersen-Mahrt, S., Durot, C., Shtrichman, R., Krainer. A.R., Kleinberger, T., Akusjaervi, G.
The adenovirus E4orf4 splicing enhancer protein interacts with a subset of hyperphosphorylated SR proteins. 2001. EMBO J. 20, 864-871.
Weisz, A., Koren, B., Cohen, T., Neufeld, G., Kleinberger, T., Lewis, B.S., and Flugelman, M.Y.
Increased vascular endothelial growth factor 165 binding to kinase insert domain-containing receptor after infection of human endothelial cells by recombinant adenovirus encoding the Vegf(165) gene. 2001. Circulation. 103, 1887-1892.
Shtrichman, R., Sharf, R., and Kleinberger, T.
Adenovirus E4orf4 protein interacts with both B alpha and B' subunits of protein phosphatase 2A, but E4orf4-induced apoptosis is mediated only by B alpha. 2000. Oncogene, 19, 3757-3765.
Kleinberger, T.
Induction of apoptosis by adenovirus E4orf4 protein. 2000. Apoptosis 5, 211-215.
Shtrichman, R., Sharf, R., Barr, H., Dobner, T., and Kleinberger, T.
Induction of apoptosis by adenovirus E4orf4 protein is specific to transformed cells and requires an interaction with protein phosphatase 2A. 1999. Proc. Natl. Acad. Sci. USA. 96, 10080-10085.
Shtrichman, R., and Kleinberger, T.
Adenovirus type 5 E4 open reading frame 4 protein induces apoptosis in transformed cells. 1998. J. Virol. 72, 2975-2982.